Substratum Stiffness and Latrunculin B Regulate Matrix Gene and Protein Expression in Human Trabecular Meshwork Cells
نویسندگان
چکیده
منابع مشابه
Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.
PURPOSE To determine the impact of substratum stiffness and latrunculin-B (Lat-B), on the expression of several matrix proteins that are associated with glaucoma. METHODS Human trabecular meshwork (HTM) cells were cultured on hydrogels possessing stiffness values mimicking those found in normal (5 kPa) and glaucomatous meshworks (75 kPa), or tissue culture polystyrene (TCP; >1 GPa). Cells wer...
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PURPOSE Extracellular matrix (ECM) composition, tension, and rigidity modulate cell-ECM interactions and have substantial impact on cell functions. The authors studied the effects of ECM rigidity on human trabecular meshwork (HTM) cells to assess ECM rigidity as a possible pathophysiologic factor in glaucoma. METHODS Trabecular meshwork cells derived from donor cornea rings and passaged three...
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PURPOSE The aim of this study was to examine the expression of genes related to the Wnt signaling pathway, such as beta-catenin (CTNNB1) and secreted frizzled-related protein-1 (sFRP1), in human trabecular meshwork (TM) cells. In addition, the effect of oxidative stress on Wnt signaling was evaluated. METHODS All experiments were conducted using second- or third-passaged human TM cells. cDNA ...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2012
ISSN: 1552-5783
DOI: 10.1167/iovs.11-8526